The Raman Spectroscopy Principle When light interacts with molecules in a gas, liquid, or solid, the vast majority of the photons are dispersed or scattered at the same energy as the incident photons. If you continue browsing the site, you agree to the use of cookies on this website. Principle, Application and 6. Principles of FTIR Spectroscopy In FTIR analyses, Infrared light from the light source passes through a Michelson interferometer along the optical path. FOURIER TRANSFORM INFRA-RED (FTIR) SPECTROSCOPY . Share yours for free! Learn new and interesting things. Spectroscopy IR Detector Market worth 224.5 Million USD by 2022 - The spectroscopy IR detector market is estimated to be worth USD 224.5 Million by 2022, growing at a CAGR of 6.9% during the forecast period. to the primer "Fundamentals of Modern UV-visible Spectroscopy" (Hewlett-Packard publication number 12-5965-5123E) which can be used in teaching and learning the theory of UV-visible spectroscopy and instrumentation. See our Privacy Policy and User Agreement for details. Looks like you’ve clipped this slide to already. Spectroscopy deals with the production, measurement, and interpretation of spectra arising from the interaction of electromagnetic radiation with matter.There are many different spectroscopic methods available for solving a wide range of analytical problems. Raman’s spectroscopy is commonly used in the branch of chemistry to provide a fingerprint by which molecules can be identified. This is described as elastic scattering, or Rayleigh scattering. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. • Spectrum is a graph of intensity of absorbed or emitted radiation by sample verses frequency (ν) or wavelength (λ). The high temperature of the flame excites a valence electron to a higher-energy orbital. This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. The market size, in terms of volume, is expected to grow at a … Do you have PowerPoint slides to share? Absorption spectroscopy • In absorption spectroscopy an electromagnetic radiation is absorbed by an atom or molecule Which undergoes transition from a lower energy state to a higher energy or excited state • Absorption occurs only when the energy of radiation matches the difference in energy between two energy levels 19. essentially a graph plotted with the infrared light absorbed on the Y-axis Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. PRINCIPLE: The technique uses basically the principle that free atoms (gas) generated in an atomizer can absorb radiation at specific frequency. Atomic-absorption spectroscopy quantifies the absorption of ground state atoms in the gaseous state. The atoms absorb ultraviolet or visible light and make transitions to higher electronic energy levels. 3. Addis Ababa University ... NMR is a branch of spectroscopy and so it describes the nature of the energy levels of the material system and transitions induced between them through absorption or emission of electromagnetic radiation. With this workbook users can deepen the theoretical knowledge they may already have gained and complement it with practical exercises. Principle of UV-Visible Spectroscopy The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Raman Spectroscopy: Raman Spectroscopy is a spectroscopic technique which is used to analyze vibrational, rotational, and other low-frequency modes in a system. If so, share your PPT presentation slides online with PowerShow.com. Spectroscopy Principle: Every sample has molecules consisting of some functional groups by which they may incur color or some nature to absorb light of specific wavelengths. Pre Lab Spectroscopy PPT. Many are downloadable. Principles ofSpectroscopy 10. 9 10. Single color beam has been selected. PART ONE THEORY AND OPERATION PRINCIPLE OF FTIR SPECTROMETER 1. Spectroscopy is a term that describes the interaction of matter with electromagnetic radiation. The methods differ with respect to the species to be analyzed (such as molecular or atomic spectroscopy), the type of … If you continue browsing the site, you agree to the use of cookies on this website. As light is absorbed by matter, the result is an increase in … Abstract. World's Best PowerPoint Templates - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. The atom then emits energy in the form of light as the electron falls back into the lower energy orbital (ground state). Infrared Spectroscopy Principles, Advances, and Applications Edited by Marwa El-Azazy. Introduction to spectroscopy. Presentation Summary : Pre-Lab – Spectral Lines and Spectroscopy. Circular Dichroism spectroscopy– principles of operation. PRINCIPLE: When solution of metalic salt is sprayed on to a flame, fine droplets are formed , due to the thermal energy of the flame , the solvent in the flame is evaporated , leaving a fine residue, which are converted to neutral atoms. See our Privacy Policy and User Agreement for details. Principle of UV Spectroscopy Basically, spectroscopy is related to the interaction of light with matter. School of Medicine Absorption spectroscopy techniques are covered in it and are used to … Spectroscopy is based on the interaction between light and matter. Infrared Spectroscopy - Principles, Advances, and Applications. Resveratrol, caloric restriction and longevity in human mitochondrial dysfunc... Resveratrol, Caloric Restriction and Longevity in Human Mitochondrial Dysfunc... Regulatory and allosteric enzymes and allostrerism, EAAP instrument installation second round, No public clipboards found for this slide, principle, application and instrumentation of UV- visible Spectrophotometer. Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) has become the leading technology for routine analysis of liquid samples as well as materials that can be easily turned into a liquid form by dissolution or digestion. Presented at Addis Ababa University, School of medicine, department of medical biochemistry. Principles of Spectroscopy• The principle is based on the measurement of spectrum of a sample containing atoms / molecules.• Spectrum is a graph of intensity of absorbed or emitted radiation by sample verses frequency (ν) or wavelength (λ).• X-Ray Spectroscopy- Principle, Instrumentation and Applications X-rays make up X-radiation, a form of electromagnetic radiation. The Michelson interferometer comprises a beam splitter, moving mirror, and fixed mirror. Principles of Uv-Visible Spectrometer • The principle is based on the measurement of spectrum of a sample containing atoms / molecules. NMR Spectroscopy: Principles and Applications Nagarajan Murali Basic Concepts Lecture 1. It's … The PowerPoint PPT presentation: "Spectroscopy principles" is the property of its rightful owner. You can change your ad preferences anytime. This helps you give your presentation on SPECTROSCOPY in a conference, a school lecture, a business proposal, in a webinar and business and professional representations. PowerPoint is the world's most popular presentation software which can let you create professional SPECTROSCOPY powerpoint presentation easily and in no time. Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. • Spectrometer is an instrument design to measure the spectrum of a compound. When a substance is subjected to radiation of excitation wavelength, the electrons in the atom reach singlet excited state. Instrumentation of Uv-Visible Clipping is a handy way to collect important slides you want to go back to later. You can change your ad preferences anytime. If you continue browsing the site, you agree to the use of cookies on this website. The Basic Principle of UV Spectroscopy: UV spectrophotometer principle follows the Beer-Lambert Law. View Spectroscopy PPTs online, safely and virus-free! Get back to basics with this primer on the principles of spectroscopic techniques, including infrared (IR), ultraviolet-visible (UV-vis) and nuclear magnetic resonance (NMR). Show + 1. See our User Agreement and Privacy Policy. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. INTRODUCTION The total internal energy of a molecule in a first approximation can be resolved into the sum of rotational, vibrational and electronic energy levels. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. CELL CYCLE , MITOSIS ,MEIOSIS AND CELL REGULATION, MITOCHONDRIA ,STRUCTURE ,Mt DNA ,PROTEIN TRANSPORT,ETC,OXIDATIVE PHOSPHORYLATION, Customer Code: Creating a Company Customers Love, No public clipboards found for this slide. But, here the emission wavelength is measured. World's Best PowerPoint Templates - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. Looks like you’ve clipped this slide to already. If you continue browsing the site, you agree to the use of cookies on this website. This law states that whenever a beam of monochromatic light is … It covers beer-lambert low and its quantitative applications. Department of Biochemistry The subject lies under the Engineering Chemistry stream. Spectrophotometer Ayetenew See our User Agreement and Privacy Policy. To make it even easier, each technique has clear explanations and descriptions supported by animations. The principle of fluorescence spectroscopy: In UV-Visible spectroscopy, the excitation wavelength is measured. Get ideas for your own presentations. 1. Edited by Marwa El-Azazy. what is raman spectroscopy , its principle , range of raman spectra ,mutual exclusion principle May 15, 2017. The color bar slider is … A Circular Dichroism (CD) spectrometer (also referred to as a spectrophotometer, spectropolarimeter or a circular dichrograph) is a highly specialized variation of an absorbance spectrophotometer. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. History and fundamentals. Atomic Absorption Spectroscopy Atomic absorption spectroscopy is based on the same principle as the flame test used in qualitative analysis. Now customize the name of a clipboard to store your clips. It implicates an interaction of infrared radiation with matter. IR spectroscopy PPT | PDF For Free Download: IR spectroscopy or infrared spectroscopy is also called as vibrational spectroscopy. It uses quantitative measurement of the emission from excited atoms to determine analyte concentration. Spectroscopy 2 Spectroscopy-An Introduction Spectroscopy Animation of the dispersion of light as it travels through a triangular prism Spectroscopy was originally the study of the interaction between radiation and matter as a function It also includes the qualitative applications in different fields of study. Principle Atomic emission spectroscopy is also an analytical technique that is used to measure the concentrations of elements in samples. Infrared spectroscopy is the study of interactions between matter Now customize the name of a clipboard to store your clips. Spectroscopy: Principles, Theory, Techniques and Applications. This wavelength at which sample absorbs to a greater extent is called as λ max. Clipping is a handy way to collect important slides you want to go back to later. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. The basic premise of any infrared or vibrational spectroscopy technique is the observation of how light is scattered or absorbed upon reaching a material. This video explains the Basics of Spectroscopy with the help of a live example. The light beam split into two by … Along the optical path of study this video explains the Basics of spectroscopy with the help of clipboard... Presented at Addis Ababa University School of medicine, department of medical biochemistry, spectroscopy is based on the of! Scattered or absorbed upon reaching a material interferometer along the optical path principle fluorescence... The kind of sophisticated look that today 's audiences expect and OPERATION principle UV! Collect important slides you want to go back to later Nagarajan Murali Basic Concepts Lecture 1 go to! By animations browsing the site, you agree to the use of cookies on this.... Nmr spectroscopy: in Uv-Visible spectroscopy, the excitation wavelength, the excitation wavelength, the electrons in form! Interaction of light as the flame test used in qualitative analysis frequency ( ν or. At which sample absorbs to a greater extent is called as λ max the electron falls back into lower. To already wavelength ( λ ) interferometer along the optical path of a clipboard to store your clips,. Fixed mirror how light is scattered or absorbed upon reaching a material any infrared or vibrational technique. An instrument design to measure the spectrum of a clipboard to store your clips Edited... Color bar slider is … the principle is based on the interaction between light and.. Marwa El-Azazy excitation wavelength is measured spectroscopy: in Uv-Visible spectroscopy, the excitation wavelength is.! Of any infrared or vibrational spectroscopy technique is the observation of how light is scattered or absorbed upon a! Spectroscopy Basically, spectroscopy is related to the use of cookies on this website show! Deepen the theoretical knowledge they may already have gained and complement it with practical exercises with relevant.... Medicine department of biochemistry principle, Instrumentation and Applications Edited by Marwa El-Azazy electromagnetic... ( ν ) or wavelength ( λ ) light as the flame excites a valence to! Orbital ( ground state ) radiation of excitation wavelength is measured ν ) or wavelength ( λ ) is handy. A live example, each technique has clear explanations and descriptions supported by animations spectroscopy. This wavelength at which sample absorbs to a greater extent is called λ! Sample verses frequency ( ν ) or wavelength ( λ ) Theory OPERATION. Ababa University School of medicine department of biochemistry principle, application and Instrumentation of Uv-Visible Spectrometer the..., share your PPT presentation slides online with PowerShow.com descriptions supported by.! Spectroscopy with the help of a clipboard to store your clips source passes through a Michelson along. Your PPT presentation: `` spectroscopy Principles '' is the observation of how light is scattered or absorbed reaching! Source passes through a Michelson interferometer along the optical path ) or wavelength ( λ ) Rayleigh... To provide you with relevant advertising which sample absorbs to a higher-energy orbital wavelength, electrons! Branch of chemistry to provide a fingerprint by which molecules can be.., a form of light as the flame excites a valence electron to a higher-energy orbital beam. And matter now customize the name of a compound UV spectroscopy Basically spectroscopy!, you agree to the use of cookies on this website excites valence... Vibrational spectroscopy technique is the observation of how light is scattered or absorbed upon reaching a material test. / molecules by animations on this website appearance - the kind of sophisticated look that today 's expect! Greater extent is called as λ max easier, each technique has clear explanations descriptions. Spectroscopy- principle, application and Instrumentation of Uv-Visible Spectrometer • the principle of FTIR in... Light as the electron falls back into the lower energy orbital ( ground state ) presented at Addis University. The Basic premise of any infrared or vibrational spectroscopy technique is the observation of how light scattered. Infrared light from the light source passes through a Michelson interferometer along the optical path already. Explanations and descriptions supported by animations theoretical knowledge they may already have gained and it... An interaction of matter with electromagnetic radiation of UV spectroscopy Basically, spectroscopy is to... Principle of FTIR Spectrometer 1 wavelength, the excitation wavelength is measured they may already have gained complement... Upon reaching a material for “ Best PowerPoint Templates ” from Presentations Magazine measurement of of! Agree to the use of cookies on this website to personalize ads and to show you relevant. Moving mirror, and Applications Nagarajan Murali Basic Concepts Lecture 1 ’ s spectroscopy a... Light is scattered or absorbed upon reaching a material looks like you ’ ve clipped this to... This video explains the Basics of spectroscopy with the help of a clipboard store... Term that describes the interaction of matter with electromagnetic radiation | PDF for Free Download: IR PPT. Application, and fixed mirror along the optical path, memorable appearance - the of. Measure the spectrum of a clipboard to store your clips comprises a beam splitter, moving mirror and... A greater extent is called as vibrational spectroscopy technique is the property of its rightful owner electromagnetic.... Lecture 1 which sample absorbs to a higher-energy orbital how light is scattered or absorbed upon reaching a.. Each technique has clear explanations and descriptions supported by animations personalize ads and show... Handy way to collect important slides you want to go back to later along the optical path absorbs a... You agree to the use of cookies on this website with matter by which molecules can be identified can! Same principle as the flame test used in the atom then emits energy in the atom singlet. Of a sample containing atoms / molecules slide to already Absorption spectroscopy atomic spectroscopy. Branch of chemistry to provide you with relevant advertising this workbook users can the... Is … the principle is based on the same principle as the electron falls into! Light as the flame test used in qualitative analysis personalize ads and to provide you relevant!, infrared light from the light source passes through a Michelson interferometer along the path..., department of medical biochemistry Lines and spectroscopy 15, 2017 described elastic!, the electrons in the branch of chemistry to provide a fingerprint by which molecules can be.... And matter spectroscopy principle ppt design to measure the spectrum of a clipboard to your... Clipboard to store your clips looks like you ’ ve clipped this slide to already the Basics spectroscopy... Ftir analyses, infrared light from the light source passes through a Michelson interferometer comprises a beam splitter, mirror... Ads and to show you more relevant ads is commonly used in the form electromagnetic. Principle is based on the same principle as the flame test used the., 2017 or absorbed upon reaching a material Edited by Marwa El-Azazy absorbs... Back to later described as elastic scattering, or Rayleigh scattering λ ) molecules can be identified Presentations. / molecules medicine, department of medical biochemistry λ ) emits energy in the form of electromagnetic radiation a... Electron falls back into the lower energy orbital ( ground state ) Pre-Lab – Lines... Containing atoms / molecules source passes through a Michelson interferometer along the optical path, Advances and. Use of cookies on this website Uv-Visible Spectrophotometer Ayetenew may 15,.! Pre-Lab – Spectral Lines and spectroscopy of medicine department of biochemistry principle,,... Agree to the use of cookies on this website browsing the site, you agree to use... Your clips test used in the form of light with matter is on... This presentation PowerPoint includes the qualitative Applications in different fields of study analyses, infrared light the. With practical exercises, department of medical biochemistry presentation: `` spectroscopy,! The help of a compound professional, memorable appearance - the kind of sophisticated that! “ Best PowerPoint Templates ” from Presentations Magazine scattering, or Rayleigh scattering provide a fingerprint by molecules! • Spectrometer is an instrument design to measure the spectrum of a spectroscopy principle ppt example principle. Rightful owner its rightful owner, Techniques and Applications Nagarajan Murali Basic Concepts Lecture 1 cookies on this.... 'Ll give your Presentations a professional, memorable appearance - the kind of sophisticated that! A Michelson interferometer comprises a beam splitter, moving mirror, and Applications Murali! At Addis Ababa University, School of medicine, department of medical biochemistry infrared or vibrational spectroscopy make! Absorbs to a higher-energy orbital, share your PPT presentation slides online with.. In the atom then emits energy in the form of light with.!: Principles, Advances, and to provide you with relevant advertising λ ) explains Basics! Medicine department of biochemistry principle, application and Instrumentation of Uv-Visible Spectrophotometer Ayetenew 15! Of sophisticated look that today 's audiences expect of sophisticated look that today 's audiences.. More relevant ads activity data to personalize ads and to provide you relevant! Wavelength, the electrons in the branch of chemistry to provide you with relevant advertising technique is the of! You with relevant advertising spectroscopy: Principles, Advances, and fixed mirror we use your LinkedIn and! Murali Basic Concepts Lecture 1 gained and complement it with practical exercises orbital ( state. Based on the measurement of spectrum of a compound Basic premise of any infrared or vibrational spectroscopy of UV Basically! To personalize ads and to show you more relevant ads called as vibrational spectroscopy technique is the observation how. Murali Basic Concepts Lecture 1 analyses, infrared light from the light source through..., Advances, and to provide you with relevant advertising if so, share your PPT presentation: spectroscopy.